Viral Culture, Cerebrospinal Fluid Related Information Synonyms Cerebrospinal Fluid for Viral Culture; CSF for Viral Culture; Viral Culture, CSF

Applies to Viral Culture, Aseptic Meningitis

Test Includes Isolation and identification of enterovirus, mumps, measles, rubella, herpes simplex virus

Laboratory Virology, BHS-03-305

Request Form Microbiology; Viral History Form

Phone (212) 305-9118

Availability Mon-Fri, 9 AM - 4 PM

Turnaround Time Positive: 3-7 days; negative: 14 days

Special Instructions
  bull Enterovirus meningitis: In addition to CSF, send throat swabs and rectal swabs in separate vials of VTM.
  bull Mumps meningitis: In addition to CSF, send throat swabs in VTM and urine in a sterile container.

Obtain Viral History Form and viral transport medium from the Virus Laboratory, BHS-03-305. Appropriate clinical history and source of specimen are essential to interpretation and should be received with the specimen. The physician's name and beeper number must be included on the request form.

Specimen Cerebrospinal fluid and additional specimens listed under Special Instructions

Volume 3 mL CSF

Minimum Volume 1 mL CSF

Storage Instructions Transport specimen to the laboratory immediately. If Virus Laboratory is not open, refrigerate at 2°C to 8°C. Do not freeze specimen.

Causes for Rejection Swab send in bacterial Culturette® not in viral transport medium, specimen leaked in transit

Reference Range Negative in tissue culture

Use Determine etiological agent of viral CNS diseases

Methodology Standard tissue culture tubes, shell vial technique with fluorescent monoclonal antibodies


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