Biopsy and Wound Culture, Mycobacteria Synonyms Acid-Fast Culture, Biopsy; AFB Culture, Biopsy; AFB Culture, Tissue; AFB Culture, Wound; Mycobacteria Culture, Biopsy; TB Culture, Biopsy; TB Culture, Tissue

Applies to Mycobacteria Culture, Surgical Specimen; Mycobacteria Culture, Tissue; Surgical Specimen Mycobacteria Culture; Tissue Mycobacteria Culture; Wound Mycobacteria Culture

Test Includes Acid-fast stain, mycobacteria culture, susceptibility on positive specimens

Laboratory Mycobacteriology Laboratory, BHS-03-312

Request Form Microbiology

Phone (212) 305-6276

Availability Daily, 8 AM - 5 PM

Turnaround Time Physician will be notified of all positive smears and cultures within 24 hours. Negative cultures are reported after 8 weeks.

Special Instructions The following information must be provided to the laboratory for the proper processing of the specimen: specific site of specimen, current antibiotic therapy, age and sex of patient, collection time and date, physician's name and pager number, and clinical diagnosis.

Specimen Tissue

Volume Small piece of tissue (as much as possible)

Container Sterile tube

Collection The portion of the surgical specimen submitted for culture should be separated from the portion submitted for histopathology by the surgeon utilizing sterile technique. Do not place in formalin.

Storage Instructions Transport specimen to the laboratory immediately. Do not store or refrigerate.

Patient Preparation Usual sterile preparation of biopsy site.

Causes for Rejection Specimen in fixative solution, specimen not received in sterile container. If an unacceptable specimen is received, the appropriate nursing station will be notified and another specimen will be requested.

Reference Range Negative for AFB

Use Isolate and identify mycobacteria; establish the etiology of granulomatous disease, fever of unknown origin (FUO) particularly in immunocompromised patients and others with defects of cellular immunity

Methodology AFB stain, culture identification and susceptibility testing when indicated


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