Alpha<sub>1</sub>-Antitrypsin Phenotyping Synonyms A-1 AT Phenotype; AAT Phenotype; AAT-Pi; Pi Phenotype; Protease Inhibitors

Test Includes Serum trypsin inhibitory capacity

Laboratory Core Laboratory

Referral to Reference Laboratory

Request Form Routine Blood Requisition

Phone (212) 305-8600

Availability Daily, 24 hours

Turnaround Time 3-5 days

Specimen Blood

Volume 4 mL

Minimum Volume 1 mL serum

Container Gold top tube

Storage Instructions Separate serum and refrigerate or freeze.

Patient Preparation Patient should be fasting.

Causes for Rejection Hemolyzed serum, specimen not refrigerated

Reference Range Interpretation accompanies report; phenotypes are designated double letters. Most common phenotypes are MM (normal), MZ (intermediate deficient), and ZZ (severe deficient).

Use Definitive diagnosis of hereditary alpha₁-antitrypsin deficiency

Methodology Isoelectric focusing